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PCR amplification: PCR was carried-out in a reaction volume of 25 m l in a 0.5 ml thin- walled PCR tube using 50 nanogram of genomic DNA as template, 1 x reaction buffer (50 mM KCl, 10 mM Tris-HCl (pH 9.0) and 0.1 Triton X-100 0.5 units of Taq polymerase (Promega 1.5 mM MgCl2, 100.

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