Participants underwent nocturnal polysomnography and reported on their sleep habits through questionnaires and sleep diaries. In addition, each morning they gave blood samples, which were evaluated for serum leptin, ghrelin, adiponectin, insulin, glucose, and lipid profile.
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To 5 a.m. (eight hours total) will have a different overall composition of sleep with more non-REM than someone who sleeps from 3 a.m. To 11 a.m. (also eight hours total) and so is likely to experience more REM.

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