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To the residue we added 3 mL of 0.2 mol/L citrate-phosphate buffer, pH 4.0. The vial was vortex-mixed until the residue was dissolved, and any emulsion was broken by centrifugation. The final stock assay solution contained 0.2 mmol/L substrate, 4 mol/L internal standard, 0.6 g/L Triton X-100, 80 mol/L acarbose, and 0.2 mol/L citrate-phosphate, pH.

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